汉族，1983年8月出生于湖南冷水江市。博士/博士后，副教授，硕士研究生导师。

2010.07—至今：桂林理工大学，化学与生物工程学院，副教授，应用化学教研室副主任

2016.03-2016.09：广西自然科学技术厅（挂职），基础研究处副处长

2014.11—2015.11：英国牛津大学（University of Oxford），化学系，博士后

2005.07—2010.12：湖南大学，化学生物传感与计量学国家重点实验室，分析化学专业，理学博士

2001.09—2005.06：湖南大学，化学化工学院，应用化学专业，理学学士

化学

生化分析及生物传感、化学计量学

1. 基于杂交链式反应和三阶校正的转录因子高灵敏荧光检测新方法研究，国家自然科学基金项目，负责人，项目批准号21565012，执行年限2016-2019；

2. 基于图案化纸和超疏水效应抑制背景信号的核酸适配体生物分析新方法研究，国家自然科学基金项目，负责人，项目批准号21205021，执行年限2013-2015；

3. 多组分荧光免疫分析结合化学计量学方法用于生物体液中多项肿瘤标记物的无干扰同时定量检测，广西自然科学基金项目，负责人，项目批准号2012GXNSFBA053029，执行年限2012-2014；

4. 基于二阶校正的三维荧光分析用于药物与DNA的相互研究，广西高等学校科研项目，负责人，项目批准号201203YB090，执行年限2012-2014；

5. 复杂体系中多维数据解析的化学计量学方法研究，桂林理工大学博士科研启动基金项目，负责人，项目批准号002401003337，执行年限2011-2014。

（一）科研工作

已在Analytical Chemistry、Chemical Communications、Biosensors and Bioelectronics、Chemometrics and Intelligent Laboratory Systems等国际权威SCI源刊发表论文近50篇，其中影响因子大于4的期刊论文22篇。论文被引次数逾700篇次，个人h-index为16。发表在国际顶级化学期刊—英国皇家化学会Chemical Communications的2篇论文均被选为“Inside Back Cover”论文，并先后被知名化学资讯平台X-MOL列为研究亮点报道。此外，作为主要成员参与译著《化学计量学实用指南》；获授权中国发明专利2项；受邀担任Journal of Chromatogrphy B、Applied Surface Science、Electrophoresis等国际重要SCI刊物的审稿专家。

（二）代表性学术论著

1. Zhang Y.,* Zhou C. B., Nie J. F.,* Le S. W., Qin Q., Liu F., Li Y. P. Equipment-free quantitative measurement for microfluidic paper-based analytical devices fabricated using the principles of movable-type printing. Analytical Chemistry (影响因子 5.886), 2014, 86(4): 2005-2012.

2. Nie J. F., Zhang Y.,* Lin L. W., Zhou C. B., Li S. H., Zhang L. M., Li J. P. Low-cost fabrication of paper-based microfluidic devices by one-step plotting. Analytical Chemistry (影响因子 5.886), 2012, 84(15): 6331-6335.

3. Nie J. F., Brown T., Zhang Y.* New two dimensional liquid-phase colorimetric assay based on old iodine–starch complexation for the naked-eye quantitative detection of analytes. Chemical Communications (影响因子 6.567), 2016, 52: 7454-7457.

Inside Back Cover论文; highlighted by知名化学资讯平台X-MOL.

4. Zhang Y.,* Yang J. N., Nie J. F.,* Yang J. H., Gao D., Zhang L., Li J. P. Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins. Chemical Communications (影响因子 6.567), 2016, 52: 3474-3477.

Inside Back Cover论文; highlighted by知名化学资讯平台X-MOL.

5. Zhang Y.,* Gao D., Fan J. L.,  Nie J. F.,* Le S. W., Zhu W. Y., Yang J. N., Li J. P. Naked-eye quantitative aptamer-based assay on paper device. Biosensors and Bioelectronics (影响因子7.476), 2016, 78: 538-546.

6. Zhang Y.,* Fan J. L.,  Nie J. F.,* Le S. W., Zhu W. Y., Gao D., Yang J. N., Zhang S. B., Li J. P. Timing readout in paper device for quantitative point-of-use hemin/G-quadruplex DNAzyme-based bioassays. Biosensors and Bioelectronics (影响因子7.476), 2015, 73: 13-18.

7. Nie J. F., Zhang Y.,* Wang H., Wang S. P., Shen G. L. Superhydrophobic surface-based magnetic electrochemical immunoassay for detection of Schistosoma japonicum antibodies. Biosensors and Bioelectronics (影响因子7.476), 2012, 33(1): 23-28.

8. Zhang Y., Wang H.,* Nie J. F., Zhang Y. W., Shen G. L.,* Yu. R. Q. Individually addressable microelectrode arrays fabricated with gold-coated pencil graphite particles for multiplexed and high sensitive impedance immunoassays. Biosensors and Bioelectronics (影响因子7.476), 2009, 25(1): 34-40.

9. Zhang Y., Wang H.,* Li J. P., Nie J. F., Zhang Y. W, Shen G. L.,* Yu. R. Q. Nitrocellulose strip array assembled on superhydrophobic surface: An aqueous solution diffusion-localized platform for multianalyte immunogold staining assays. Biosensors and Bioelectronics (影响因子7.476), 2011, 26 (7): 3272-3277.

10. Nie J. F.,* Li B., Zhang Y.,* Fan J. L., Yi Z. S., Cai Z. R. High-order calibration for the spectrofluorimetric determination of pesticides based on photochemical derivatization. A solution of the problems of inner-filter effects and matrix interferences in complex environmental water. Chemometrics and Intelligent Laboratory Systems, 2016, 156: 36-53.

11. Nie J. F., Zhang Y.,* Yi Z. S., Zou H. Y., Fu H. Y., Wu H. L. Advanced chemometric algorithm coupled to subspace projection technique between original and pseudo samples: A snapshot of chemical rank estimation for high-order data. Chemometrics and Intelligent Laboratory Systems, 2012, 118: 211-218.

12. Nie J. F., Wu H. L.,* Wang J. Y., Liu Y. J., Yu. R. Q. Chemical rank estimation for excitation-emission matrix fluorescence data by region-based moving window subspace projection technique and Monte Carlo simulation. Chemometrics and Intelligent Laboratory Systems, 2010, 104: 271-280.

13. Nie J. F., Liang Y. Z., Zhang Y.,* Le S. W., Li D. N., Zhang S. B. One-step patterning of hollow microstructures in paper by laser cutting to create microfluidic analytical devices. Analyst (影响因子4.033), 2013, 138(2): 671-676.

14. Zhang Y.,* Liu F., Nie J. F.,* Jiang F. Y., Zhou C. B., Yang J. N., Fan J. L., Li J. P. An electrochemical sensing platform based on local repression of electrolyte diffusion for single-step, reagentless, sensitive detection of a sequencespecific DNA-binding protein. Analyst (影响因子4.033), 2014, 139(9): 193-2198.

15. Nie J. F., Wu H. L.*, Wang X. M., Zhang Y., Zhu S. H., Yu R. Q. Determination of testosterone propionate in cosmetics using excitation-emission matrix fluorescence based on oxidation derivatization with the aid of second-order calibration methods. Analytica Chimica Acta (影响因子4.712), 2008, 628: 24-32.

16. Nie J. F., Wu H. L.*, Zhu S. H., Han Q. J., Fu H. Y., Li S. F., Yu R. Q. Simultaneous determination of 6-methylcoumarin and 7-methoxycoumarin in cosmetics using three-dimensional excitation–emission matrix fluorescence coupled with second-order calibration methods. Talanta (影响因子4.035), 2008, 75: 1260-1269.

17. Nie J. F., Wu H. L.*, Zhang S. R., Yu Y. J., Yu R. Q. Self-weighted alternating normalized residue fitting algorithm with application to quantitative analysis of excitation-emission matrix fluorescence data. Analytical Methods, 2010, 2: 1918-1926.

18. Nie J. F., Wu H. L.*, Xia A. L., Zhu S. H., Bian Y. C., Li S. F., Yu R. Q., Determination of sulpiride in human urine using excitation-emission matrix fluorescence coupled with second-order calibration. Analytical Sciences, 2007, 23: 1377-1382.

19. 聂瑾芳, 吴海龙*, 张云, 刘亚娟, 王建瑶, 李勇, 俞汝勤. 三线性分解方法用于激发-发射矩阵荧光数据中一阶瑞利散射的扣除. 中国科学: 化学, 2011 , 41(11): 1740-1749.

20. Wu H. L.*, Nie J. F., Yu Y. J., Yu. R. Q. Multi-way chemometric methodologies and applications: A central summary of our research work (邀请综述). Analytica Chimica Acta (影响因子4.712), 2009, 650: 131-142.

21. Zhang Y., Wang H.,* Nie J. F., Zhou H., Shen G. L.*, Yu R. Q. Mussel-inspired fabrication of encoded polymer films for electrochemical identification. Electrochemistry Communication (影响因子4.569), 2009, 11: 1936-1939.

22. Yu Y. J., Wu H. L.*, Nie J. F., Zhang S. R., Li S. F., Li Y. N., Zhu S. H., Yu R. Q. A comparison of several trilinear second-order calibration algorithms. Chemometrics and Intelligent Laboratory Systems, 2011, 106: 93-107.

23. OuYang L. Q., Wu H. L.*, Nie J. F., Zhang Y., Zou H. Y., Fu H. Y., Yu R. Q. Simultaneous determination of psoralen and isopsoralen in plasma and Chinese medicine Xian Ling Gu Bao capsule by using HPLC-DAD coupled with alternating trilinear decomposition algorithm. Analytica Chimica Acta (影响因子4.712), 2009, 650: 160-166.

24. Zou H. Y., Wu H. L.*, OuYang L. Q., Zhang Y., Nie J. F., Fu H. Y., Yu R. Q. Fluorescent quantification of terazosin hydrochloride content in human plasma and tablets using second-order calibration based on both parallel factor analysis and alternating penalty trilinear decomposition. Analytica Chimica Acta (影响因子4.712), 2009, 650: 143-149.

25. Fu H. Y., Wu H. L.*, Yu Y. J., Yu L. L., Zhang S. R., Nie J. F., Li S. F., Yu R. Q. A new third-order calibration method with application for analysis of four-way data arrays. Journal of Chemometrics, 2011, 25: 408-429.

26. Li S. F., Wu H. L.*, Yu Y. J., Li Y. N., Nie J. F., Fu H. Y., Yu R. Q. Quantitative analysis of levodopa, carbidopa and methyldopa in human plasma samples using HPLC-DAD combined with second-order calibration based on alternating trilinear decomposition algorithm. Talanta (影响因子4.035), 2010, 81: 805-812.

27. Cai C. B., Xu L., Han Q. J., Wu H. L., Nie J. F., Fu H. Y., Yu R. Q.* Combining the least correlation design, wavelet packet transform and correlation coefficient test to reduce the size of calibration set for NIR quantitative analysis in multi-component systems. Talanta (影响因子4.035), 2010, 81: 799-804.

28. Cai C. B., Han Q. J., Tang L. J., Nie J. F., OuYang L. Q., Yu R. Q.* Treating NIR data with orthogonal discrete wavelet transform: Predicting concentrations of a multi-component system through a small-scale calibration set. Talanta (影响因子4.035), 2008, 77: 822-826.

29. Zhu S. H., Wu H. L.*, Xia A. L., Nie J. F., Bian Y. C., Cai C. B., Yu R. Q, Excitation-emission-kinetic fluorescence coupled with third-order calibration for quantifying carbaryl and investigating the hydrolysis in effluent water. Talanta (影响因子4.035), 2009, 77: 1640-1646.

30. Zou H. Y., Wu H. L.*, Fu H. Y., Tang L. J., Xu L., Nie J. F., Yu R. Q. Variable-weighted least-squares support vector machine for multivariate spectral analysis. Talanta (影响因子4.035, 2010, 80: 1698-1701.

31. Wang X. M., Wu H. L.*, Nie J. F., Li Y. N., Yu Y. J., Yu R. Q. Rapid determination of thiabendazole in orange extract using excitation-emission matrix fluorescence and second-order calibration based on alternating trilinear decomposition/alternating normalization-weighted error algorithms. Science in China, Series B: Chemistry, 2008, 51: 729-735.

32. 王雪梅, 吴海龙*, 聂瑾芳, 李元娜, 于永杰, 俞汝勤. 激发发射荧光二阶张量校正算法快速检测桔子中噻苯咪唑. 中国科学B, 2008, 38: 288-293.

33. Wang X. M., Wu H. L.,* Nie J. F., Yu R. Q. Rapid determination of bitertanol content in banana using three-dimensional fluorescence coupled with second-order calibration. Chinese Journal of Analytical Chemistry, 2009, 37: 811-816. 

34. Han Q. J., Wu H. L.,* Nie J. F., Xia A L., Zhu S. H., Yu R. Q. Direct determination of reserpine in urine using excitation-emission fluorescence combined with three-way chemometric calibration methodologies. Frontiers of Chemistry in China-Selected Publications from Chinese Universities, 2008, 3: 224-228.

35. Wang X. M., Wu H. L.,* Nie J. F., Li Y. N., Yu Y. J., Yu R. Q. Rapid determination of thiabendazole in orange extract using excitation-emission matrix fluorescence and second-order calibration based on alternating trilinear decomposition/alternating normalization-weighted error algorithms. Science in China, Series B: Chemistry, 2008, 51: 729-735.

36. Li Y. N., Wu H. L.,* Nie J. F., Li S. F., Yu Y. J., Zhang S. R., Yu R. Q. Interference-free determination of abscisic acid and gibberellin in plant samples by using excitation-emission matrix fluorescence based on oxidation derivatization coupled with second-order calibration methods. Analytical Methods, 2009, 1: 115-122.

37. Zou H. Y., Wu H. L.,* Li Y. N., Nie J. F., Fu H. Y., Li S. F., Ouyang L. Q., Yu R. Q. Simultaneous determination of dextromethorphan and quinidine in biological fluids by excitation-emission matrix fluorescence coupled with second-order calibration methods. Analytical Letters, 2010, 43, 2739-2750.

38. Li S. F., Wu H. L.,* Huang L., Li Y. N., Nie J. F., Zhang S. R., Yu R. Q. Quantitative analysis of fluphenazine hydrochloride in human urine samples using excitation-emission matrix fluorescence based on oxidation derivation and combined with second-order calibration methods. Analytical Methods, 2010, 2, 1069-1077.

39. Li Y. N., Wu H. L.,* Zhang S. R., Nie J. F., Yu Y. J., Wang X. M., Yu R. Q. Determination of indole-3-Acetic acid in soil using excitation-emission matrix fluorescence with trilinear decomposition-based calibration methods. Analytical Sciences, 2009, 25: 83-88.  

40. Zou H. Y., Wu H. L.,* Zhang Y., Li S. F., Nie J. F., Fu H. Y., Yu R. Q. Studying the interaction of pirarubicin with DNA and determining pirarubicin in human urine samples: Combining excitation-emission fluorescence matrices with second-order calibration methods. Journal of Fluorescence, 2009, 6: 955-966.

41. Li S. F., Wu H. L.,* Xia A L., Zhu S. H., Nie J. F., Yu R. J., Yu R. Q. Quantitative analysis of epinephrine in human plasma samples using kinetic fluorometric method combined with second-order calibration. Analytical Sciences, 2009, 25: 1231-1236.

42. Fu H. Y., Wu H. L.,* Zou H. Y., Tang L. J., Xu L, Cai C. B., Nie J. F., Yu R. Q. Automatic configuration of optimized sample-weighted least-squares support vector machine by particle swarm optimization for multivariate spectral analysis. Analytical Methods, 2010, 2: 282-288.

43. Shao S. Z., Wu H. L.,* Li S. F., Nie J. F., Wang K. Y., Liang Y., Yu R. Y. Determination of levodopa in both human Urine and plasma samples using excitation–emission matrix fluorescence coupled with second-order calibration. Journal of Analytical Science, 2012, 24: 373-378.

44. Liu Y. J., Wu H. L.,* Kang C., Gu H. W., Nie J. F., Li S. S., Su Z. Y., Yu R. Q. Four-way self-weighted alternating normalized residue fitting algorithm with application for the analysis of serotonin in human plasma. Analytical Sciences, 2011, 28(11): 1097-1104.

45. Fu H. Y., Wu H. L.,* Nie J. F., Yu Y. J., Zou H.Y., Yu R. Q. Highly sensitive fluorescence quantification of irinotecan in biological fluids with the aid of second-order advantage. Chinese Chemical Letters, 2010, 21(12): 1482-1486.

（三）获奖情况

1. 教学/教改获奖

获第十一届全国多媒体课件大赛优秀奖；

获第十二届全国多媒体课件大赛优秀奖；

获第八届广西高校教学软件大赛优秀奖；

获第十二届广西区教育教学软件大赛二等奖；

获桂林理工大学2013年“优秀青年授课老师”荣誉称号；

获桂林理工大学2013年首届微课教学比赛二等奖；

获桂林理工大学2014年“优秀班导师”荣誉称号；

获桂林理工大学第二届多媒体教学软件比赛二等奖；

获桂林理工大学第三届多媒体教学软件比赛二等奖。

2. 指导本科生/研究生获奖

本科生李佐获2015年广西高校化学化工类论文及设计竞赛一等奖；

硕士生周财斌获2012、2013和2014年桂林理工大学二等奖学金，被评为2014年桂林理工大学优秀毕业生、广西区优秀毕业生；

硕士生范金龙等获“第二届优利特杯科技创新设计大赛”一等奖；

硕士生刘芳获2014年桂林理工大学校级三等奖学金。

（四）新闻报道

1. X-MOL: 基于笔式pH计的新型ELISA, http://www.x-mol.com/news/2020；

2. X-MOL: 二维液相比色分析新理论: 首次实现液相中分析物免仪器定量检测, http://www.x-mol.com/news/3067。

《分子计算模拟》