方维焕简历

 

姓    名：方维焕

性    别：男

联系方式：电话：88982242，传真：88982242

电子邮件：whfang@zju.edu.cn

实验室网页：http://www.molmicro-foodsafety.net/

学  历：

1993.11 - 1996.06：芬兰赫尔辛基大学兽医学院 哲学博士

1982.02 - 1985.01：浙江农业大学兽医系 硕士

1978.03 - 1982.01：浙江农业大学兽医系 学士

工作简历：

1996.12 - 至今：浙江大学动物科学学院 教授

2006.09 - 2006.12：美国哈佛大学医学院微生物与分子遗传系 访问学者

2001.07 - 2001.10：丹麦皇家兽医与农业大学 访问学者

1996.12 - 1998.12：美国田纳西大学动物科学系 博士后

1992.10 - 1996.11：浙江农业大学动物科学学院 副教授

1988.04 - 1992.09：浙江农业大学兽医系 讲师

1985.02 - 1988.03：浙江农业大学兽医系 助教

兼  职：

2011 - 至今：International Committee on Food Microbiology and Hygiene执行委会会委员(Executive Board Member)

2008 -至今：中国畜牧兽医学会常务理事

2008 -至今：浙江省畜牧兽医学会 常务副理事长

2007 -至今：Foodborne Pathogens and Disease 编委

2005 -至今：中国畜牧兽医学会兽医食品卫生学分会 副理事长

2005 -至今：《微生物学报》编委

2005 -至今：《中国兽医科学》编委

2004 -至今：浙江省动物预防医学重点实验室 主任

2002 -至今：International Journal of Food Microbiology 编委

2000 -至今：浙江大学动物预防医学研究所 所长

1999 -至今：《畜牧兽医学报》编委

2008 - 2011：International Journal of Food Microbiology  编辑

1999 - 2009：浙江大学动物科学学院 副院长

研究方向：

（1）动物疫病和食源性病原感染生物学

（2）微生物性食品安全

学术论文（英文）：

[1] Zhou, Y., Qi, B., Gu, Y., Xu, F., Du, H., Li, X., and Fang, W. (2016) Porcine Circovirus 2 Deploys PERK Pathway and GRP78 for Its Enhanced Replication in PK-15 Cells, Viruses 8.

[2] Zhou, Y., Liang, Q., Li, W., Gu, Y., Liao, X., Fang, W., and Li, X. (2016) Characterization and functional analysis of toll-like receptor 4 in Chinese soft-shelled turtle Pelodiscus sinensis, Dev Comp Immunol 63, 128-135.

[3] Xia, Y., Xin, Y., Li, X., and Fang, W. (2016) To Modulate Survival under Secondary Stress Conditions, Listeria monocytogenes 10403S Employs RsbX To Downregulate sigmaB Activity in the Poststress Recovery Stage or Stationary Phase, Appl Environ Microbiol 82, 1126-1135.

[4] Wang, X., Shao, C., Wang, L., Li, Q., Song, H., and Fang, W. (2016) The viral non-structural protein 1 alpha (Nsp1alpha) inhibits p53 apoptosis activity by increasing murine double minute 2 (mdm2) expression in porcine reproductive and respiratory syndrome virus (PRRSV) early-infected cells, Vet Microbiol 184, 73-79.

[5] Shan, Y., Zhang, Y., Zhuo, X., Li, X., Peng, J., and Fang, W. (2016) Matrix metalloproteinase-9 plays a role in protecting zebrafish from lethal infection with Listeria monocytogenes by enhancing macrophage migration, Fish Shellfish Immunol 54, 179-187.

[6] Liao, X., Wang, Z., Cao, T., Tong, C., Geng, S., Gu, Y., Zhou, Y., Li, X., and Fang, W. (2016) Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization, Vaccine.

[7] Liang, Q., Li, W., Guo, N., Tong, C., Zhou, Y., Fang, W., and Li, X. (2016) Identification and Functional Analysis of Interleukin-1beta in the Chinese Soft-Shelled Turtle Pelodiscus sinensis, Genes (Basel) 7.

[8] Jiang, X., Yang, Y., Zhu, L., Gu, Y., Shen, H., Shan, Y., Li, X., Wu, J., and Fang, W. (2016) Live Streptococcus suis type 5 strain XS045 provides cross-protection against infection by strains of types 2 and 9, Vaccine.

[9] Gu, Y., Qi, B., Zhou, Y., Jiang, X., Zhang, X., Li, X., and Fang, W. (2016) Porcine Circovirus Type 2 Activates CaMMKbeta to Initiate Autophagy in PK-15 Cells by Increasing Cytosolic Calcium, Viruses 8.

[10] Fang, C., Shan, Y., Cao, T., Xia, Y., Xin, Y., Cheng, C., Song, H., Li, X., and Fang, W. (2016) Prevalence and Virulence Characterization of Listeria monocytogenes in Chilled Pork in Zhejiang Province, China, Foodborne Pathog Dis 13, 8-12.

[11] Fang, C., Cao, T., Shan, Y., Xia, Y., Xin, Y., Cheng, C., Song, H., Bowman, J., Li, X., Zhou, X., and Fang, W. (2016) Comparative Genomic Analysis Reveals That the 20K and 38K Prophages in Listeria monocytogenes Serovar 4a Strains Lm850658 and M7 Contribute to Genetic Diversity but Not to Virulence, J Microbiol Biotechnol 26, 197-206.

[12] Zhang, X., Wang, X., Sun, M., Zhang, X., Song, H., Yan, Y., Sun, J., Li, X., and Fang, W. (2015) A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples, Toxins 7, 4216-4231.

[13] Zhang, X., Sun, M., Kang, Y., Xie, H., Wang, X., Song, H., Li, X., and Fang, W. (2015) Identification of a high-affinity monoclonal antibody against ochratoxin A and its application in enzyme-linked immunosorbent assay, Toxicon 106, 89-96.

[14] Zhang, X., Li, K., Wu, S., Shuai, J., and Fang, W. (2015) Peptide nucleic acid fluorescence in-situ hybridization for identification of Vibrio spp. in aquatic products and environments, Int J Food Microbiol 206, 39-44.

[15] Zhang, X., Jiang, X., Yang, L., Fang, L., Shen, H., Lu, X., and Fang, W. (2015) DnaJ of Streptococcus suis Type 2 Contributes to Cell Adhesion and Thermotolerance, J Microbiol Biotechnol 25, 771-781.

[16] Yu, Y., Fang, L., Zhang, Y., Sheng, H., and Fang, W. (2015) VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages, Front Microbiol 6, 168.

[17] Wang, Y. W., Yue, H., Fang, W., and Huang, Y. W. (2015) Complete Genome Sequence of Porcine Deltacoronavirus Strain CH/Sichuan/S27/2012 from Mainland China, Genome announcements 3.

[18] Tong, C., Chen, N., Liao, X., Xie, W., Li, D., Li, X., and Fang, W. (2015) The Epitope Recognized by Monoclonal Antibody 2B6 in the B/C Domains of Classical Swine Fever Virus Glycoprotein E2 Affects Viral Binding to Hyperimmune Sera and Replication, J Microbiol Biotechnol 25, 537-546.

[19] Shan, Y., Fang, C., Cheng, C., Wang, Y., Peng, J., and Fang, W. (2015) Immersion infection of germ-free zebrafish with Listeria monocytogenes induces transient expression of innate immune response genes, Front Microbiol 6, 373.

[20] Fang, L., Shen, H., Tang, Y., and Fang, W. (2015) Superoxide dismutase of Streptococcus suis serotype 2 plays a role in anti-autophagic response by scavenging reactive oxygen species in infected macrophages, Vet Microbiol 176, 328-336.

[21] Fang, C., Cao, T., Cheng, C., Xia, Y., Shan, Y., Xin, Y., Guo, N., Li, X., Song, H., and Fang, W. (2015) Activation of PrfA results in overexpression of virulence factors but does not rescue the pathogenicity of Listeria monocytogenes M7, J Med Microbiol 64, 818-827.

[22] Cheng, C., Yang, Y., Dong, Z., Wang, X., Fang, C., Yang, M., Sun, J., Xiao, L., Fang, W., and Song, H. (2015) Listeria monocytogenes varies among strains to maintain intracellular pH homeostasis under stresses by different acids as analyzed by a high-throughput microplate-based fluorometry, Front Microbiol 6, 15.

[23] Cheng, C., Wang, X., Dong, Z., Shao, C., Yang, Y., Fang, W., Fang, C., Wang, H., Yang, M., Jiang, L., Zhou, X., and Song, H. (2015) Aminopeptidase T of M29 Family Acts as A Novel Intracellular Virulence Factor for Listeria monocytogenes Infection, Sci Rep 5, 17370.

[24] Wen, G., Zhang, Y., Zhang, X., Hu, H., Zhang, H., Cheng, C., Wang, X., Li, X., and Fang, W. (2013) Functional characterization of porcine LSm14A in IFN-beta induction, Vet Immunol Immunopathol 155, 110-116.

[25] Wang, W., Li, M., Fang, W., Pradhan, A. K., and Li, Y. (2013) A predictive model for assessment of decontamination effects of lactic acid and chitosan used in combination on Vibrio parahaemolyticus in shrimps, Int J Food Microbiol 167, 124-130.

[26] Shuai, J., Zhang, X., Chen, W., Li, K., Wu, S., He, Y., and Fang, W. (2013) In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS), Virology journal 10, 16.

[27] Liu, Y., Fang, C., Cheng, C., and Fang, W. (2013) [Inactivation kinetics of Vibrio parahaemolyticus in peeled shrimp treated with organic acids], Wei Sheng Wu Xue Bao 53, 31-37.

[28] Li, M., Wang, W., Fang, W., and Li, Y. (2013) Inhibitory effects of chitosan coating combined with organic acids on Listeria monocytogenes in refrigerated ready-to-eat shrimps, J Food Prot 76, 1377-1383.

[29] Hu, H., Zhang, X., Zhang, H., Wen, G., Zhang, Q., Li, X., and Fang, W. (2013) Porcine reproductive and respiratory syndrome virus inhibition of interferon-beta transcription by IRF3-independent mechanisms in MARC-145 cells in early infection, Vet Immunol Immunopathol 156, 135-140.

[30] Cheng, C., Chen, J., Shan, Y., Fang, C., Liu, Y., Xia, Y., Song, H., and Fang, W. (2013) Listeria monocytogenes ArcA contributes to acid tolerance, J Med Microbiol 62, 813-821.

[31] Cheng, C., Chen, J., Fang, C., Xia, Y., Shan, Y., Liu, Y., Wen, G., Song, H., and Fang, W. (2013) Listeria monocytogenes aguA1, but not aguA2, encodes a functional agmatine deiminase: biochemical characterization of its catalytic properties and roles in acid tolerance, J Biol Chem 288, 26606-26615.

[32] Chen, J., Cheng, C., Lv, Y., and Fang, W. (2013) Genetic diversity of internalin genes in the ascB-dapE locus among Listeria monocytogenes lineages III and IV strains, J Basic Microbiol 53, 778-784.

[33] Chen, J., Chen, F., Cheng, C., and Fang, W. (2013) Prevalence of the lmo0036-0043 gene cluster encoding arginine deiminase and agmatine deiminase systems in Listeria monocytogenes, New Microbiol 36, 187-192.

[34] Zhu, B., Zhou, Y., Xu, F., Shuai, J., Li, X., and Fang, W. (2012) Porcine circovirus type 2 induces autophagy via the AMPK/ERK/TSC2/mTOR signaling pathway in PK-15 cells, J Virol 86, 12003-12012.

[35] Zhu, B., Xu, F., Li, J., Shuai, J., Li, X., and Fang, W. (2012) Porcine circovirus type 2 explores the autophagic machinery for replication in PK-15 cells, Virus Res 163, 476-485.

[36] Zhang, X., Wu, S., Li, K., Shuai, J., Dong, Q., and Fang, W. (2012) Peptide nucleic acid fluorescence in situ hybridization for identification of Listeria genus, Listeria monocytogenes and Listeria ivanovii, Int J Food Microbiol 157, 309-313.

[37] Yu, Y., Zhang, Y., Li, J., Yang, H., Song, H., and Fang, W. (2012) [VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2], Wei Sheng Wu Xue Bao 52, 954-961.

[38] Yu, Y., Yang, H., Li, J., Zhang, P., Wu, B., Zhu, B., Zhang, Y., and Fang, W. (2012) Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers, Arch Microbiol 194, 827-835.

[39] Wu, X., Fang, W., Yu, Y., and Song, H. (2012) [Development and application of homologous recombination knockout system in Mycobacterium tuberculosis], Wei Sheng Wu Xue Bao 52, 1151-1159.

[40] Tang, Y., Zhang, X., Wu, W., Lu, Z., and Fang, W. (2012) Inactivation of the sodA gene of Streptococcus suis type 2 encoding superoxide dismutase leads to reduced virulence to mice, Vet Microbiol 158, 360-366.

[41] Tang, Y., Wu, W., Zhang, X., Lu, Z., Chen, J., and Fang, W. (2012) Catabolite control protein A of Streptococcus suis type 2 contributes to sugar metabolism and virulence, J Microbiol 50, 994-1002.

[42] Li, X. L., Kang, Y., Zhang, X. Y., Zhu, B. L., and Fang, W. H. (2012) Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress, J Zhejiang Univ Sci B 13, 465-477.

[43] Huang, L., Fang, W., Yu, Y., and Song, H. (2012) [Membrane protein characterization by photoactivatable localization microscopy], Sheng Wu Gong Cheng Xue Bao 28, 1378-1387.

[44] Chen, J., Fang, C., Zhu, N., Lv, Y., Cheng, C., Bei, Y., Zheng, T., and Fang, W. (2012) Genetic organization of ascB-dapE internalin cluster serves as a potential marker for Listeria monocytogenes sublineages IIA, IIB, and IIC, J Microbiol Biotechnol 22, 575-584.

[45] Chen, J., Fang, C., Zheng, T., Zhu, N., Bei, Y., and Fang, W. (2012) Genomic presence of gadD1 glutamate decarboxylase correlates with the organization of ascB-dapE internalin cluster in Listeria monocytogenes, Foodborne Pathog Dis 9, 175-178.

[46] Zhao, H., Chen, J., Fang, C., Xia, Y., Cheng, C., Jiang, L., and Fang, W. (2011) Deciphering the biodiversity of Listeria monocytogenes lineage III strains by polyphasic approaches, J Microbiol 49, 759-767.

[47] Zhang, P., Liu, Y., Fang, C., Yu, Y., Chen, J., and Fang, W. (2011) [Comparison of growth models of Listeria monocytogenes in chilled pork], Wei Sheng Wu Xue Bao 51, 1625-1631.

[48] Yu, Y., Hu, W., Wu, B., Zhang, P., Chen, J., Wang, S., and Fang, W. (2011) Vibrio parahaemolyticus isolates from southeastern Chinese coast are genetically diverse with circulation of clonal complex 3 strains since 2002, Foodborne Pathog Dis 8, 1169-1176.

[49] Tang, Y., Zhao, H., Wu, W., Wu, D., Li, X., and Fang, W. (2011) Genetic and virulence characterization of Streptococcus suis type 2 isolates from swine in the provinces of Zhejiang and Henan, China, Folia Microbiol (Praha) 56, 541-548.

[50] Shuai, J., Fu, L., Zhang, X., Zhu, B., Li, X., He, Y., and Fang, W. (2011) Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro: Implications for the role of NLS in viral replication, Virology journal 8, 341.

[51] Li, X., Zhu, B., Chen, N., Hu, H., Chen, J., Zhang, X., Li, J., and Fang, W. (2011) Molecular characterization and functional analysis of MyD88 in Chinese soft-shelled turtle Trionyx sinensis, Fish Shellfish Immunol 30, 33-38.

[52] Chen, J., Xia, Y., Cheng, C., Fang, C., Shan, Y., Jin, G., and Fang, W. (2011) Genome sequence of the nonpathogenic Listeria monocytogenes serovar 4a strain M7, J Bacteriol 193, 5019-5020.

[53] Chen, J., Cheng, C., Xia, Y., Zhao, H., Fang, C., Shan, Y., Wu, B., and Fang, W. (2011) Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance, Microbiology 157, 3150-3161.

[54] Bai, F., Chen, J., Chen, Q., Luo, X., Fang, W., and Jiang, L. (2011) A single substitution in 5"-untranslated region of plcB is involved in enhanced broad-range phospholipase C activity in Listeria monocytogenes strain H4, Acta Biochim Biophys Sin (Shanghai) 43, 275-283.

[55] Jiang, L., Olesen, I., Andersen, T., Fang, W., and Jespersen, L. (2010) Survival of Listeria monocytogenes in simulated gastrointestinal system and transcriptional profiling of stress- and adhesion-related genes, Foodborne Pathog Dis 7, 267-274.

[56] Chen, N., Tong, C., Li, D., Wan, J., Yuan, X., Li, X., Peng, J., and Fang, W. (2010) Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China, Virology journal 7, 378.

[57] Chen, N., Li, D., Yuan, X., Li, X., Hu, H., Zhu, B., Wan, X., and Fang, W. (2010) Genetic characterization of E2 gene of classical swine fever virus by restriction fragment length polymorphism and phylogenetic analysis, Virus Genes 40, 389-396.

[58] Chen, J., Chen, Q., Jiang, L., Cheng, C., Bai, F., Wang, J., Mo, F., and Fang, W. (2010) Internalin profiling and multilocus sequence typing suggest four Listeria innocua subgroups with different evolutionary distances from Listeria monocytogenes, BMC Microbiol 10, 97.

[59] Chen, J., Chen, Q., Jiang, J., Hu, H., Ye, J., and Fang, W. (2010) Serovar 4b complex predominates among Listeria monocytogenes isolates from imported aquatic products in China, Foodborne Pathog Dis 7, 31-41.

[60] Yu, Y., Wu, B., and Fang, W. (2009) [Type III secretion system of Vibrio parahaemolyticus--a review], Wei Sheng Wu Xue Bao 49, 848-852.

[61] Wang, S., Vongxay, K., Shen, B., Zhou, X., Jin, P., Chen, J., and Fang, W. (2009) [Analysis of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods], Wei Sheng Wu Xue Bao 49, 1576-1583.

[62] Liang, Q., Chen, J., Chen, Q., Lao, H., Huang, S., and Fang, W. (2009) [Molecular epidemiology of Listeria monocytogenes isolates from imported fishery products to China], Wei Sheng Wu Xue Bao 49, 766-772.

[63] Hu, H., Li, X., Zhang, Z., Shuai, J., Chen, N., Liu, G., and Fang, W. (2009) Porcine reproductive and respiratory syndrome viruses predominant in southeastern China from 2004 to 2007 were from a common source and underwent further divergence, Arch Virol 154, 391-398.

[64] Chen, X., Chen, Y., Li, X., Chen, N., and Fang, W. (2009) Characterization of surface layer proteins in Lactobacillus crispatus isolate ZJ001, J Microbiol Biotechnol 19, 1176-1183.

[65] Chen, J., Zhang, X., Mei, L., Jiang, L., and Fang, W. (2009) Prevalence of Listeria in Chinese food products from 13 provinces between 2000 and 2007 and virulence characterization of Listeria monocytogenes isolates, Foodborne Pathog Dis 6, 7-14.

[66] Chen, J., Luo, X., Jiang, L., Jin, P., Wei, W., Liu, D., and Fang, W. (2009) Molecular characteristics and virulence potential of Listeria monocytogenes isolates from Chinese food systems, Food Microbiol 26, 103-111.

[67] Chen, J., Jiang, L., Chen, X., Luo, X., Chen, Y., Yu, Y., Tian, G., Liu, D., and Fang, W. (2009) Listeria monocytogenes serovar 4a is a possible evolutionary intermediate between L. monocytogenes serovars 1/2a and 4b and L. innocua, J Microbiol Biotechnol 19, 238-249.

[68] Chen, J., Jiang, L., Chen, Q., Zhao, H., Luo, X., Chen, X., and Fang, W. (2009) lmo0038 is involved in acid and heat stress responses and specific for Listeria monocytogenes lineages I and II, and Listeria ivanovii, Foodborne Pathog Dis 6, 365-376.

[69] Yang, Z. Z., Shuai, J. B., Dai, X. J., and Fang, W. H. (2008) A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China, J Zhejiang Univ Sci B 9, 148-153.

[70] Vongxay, K., Wang, S., Zhang, X., Wu, B., Hu, H., Pan, Z., Chen, S., and Fang, W. (2008) Pathogenetic characterization of Vibrio parahaemolyticus isolates from clinical and seafood sources, Int J Food Microbiol 126, 71-75.

[71] Vongxay, K., Pan, Z., Zhang, X., Wang, S., Cheng, S., Mei, L., Xu, C., and Fang, W. (2008) Occurrence of pandemic clones of Vibrio parahaemolyticus isolates from seafood and clinical samples in a Chinese coastal province, Foodborne Pathog Dis 5, 127-134.

[72] Shuai, J., Wei, W., Jiang, L., Li, X., Chen, N., and Fang, W. (2008) Mapping of the nuclear localization signals in open reading frame 2 protein from porcine circovirus type 1, Acta Biochim Biophys Sin (Shanghai) 40, 71-77.

[73] Shuai, J., Li, X., Chen, N., Chen, X., and Fang, W. (2008) Characterization and potential use of truncated PCV2 capsid protein and its polyclonal antibody for diagnosis of PCV2 infections, Wei Sheng Wu Xue Bao 48, 85-90.

[74] Li, X. L., Zhang, C. L., Fang, W. H., and Lin, F. C. (2008) White-spot disease of Chinese soft-shelled turtles (Trionyx sinens) caused by Paecilomyces lilacinus, J Zhejiang Univ Sci B 9, 578-581.

[75] Jiang, L., Chen, J., Xu, J., Zhang, X., Wang, S., Zhao, H., Vongxay, K., and Fang, W. (2008) Virulence characterization and genotypic analyses of Listeria monocytogenes isolates from food and processing environments in eastern China, Int J Food Microbiol 121, 53-59.

[76] Chen, N., Hu, H., Zhang, Z., Shuai, J., Jiang, L., and Fang, W. (2008) Genetic diversity of the envelope glycoprotein E2 of classical swine fever virus: recent isolates branched away from historical and vaccine strains, Vet Microbiol 127, 286-299.

[77] Yang, Z. Z., Habib, M., Shuai, J. B., and Fang, W. H. (2007) Detection of PCV2 DNA by SYBR Green I-based quantitative PCR, J Zhejiang Univ Sci B 8, 162-169.

[78] Yang, Z., Chen, A., Sun, H., Ye, Y., and Fang, W. (2007) Ginsenoside Rd elicits Th1 and Th2 immune responses to ovalbumin in mice, Vaccine 25, 161-169.

[79] Shuai, J., Wei, W., Li, X., Chen, N., Zhang, Z., Chen, X., and Fang, W. (2007) Genetic characterization of porcine circovirus type 2 (PCV2) from pigs in high-seroprevalence areas in southeastern China, Virus Genes 35, 619-627.

[80] Pan, Z. J., Vongxay, K., Shen, B., Lou, G. M., and Fang, W. H. (2007) [Analysis of major phenotypes and hemolysin-related genotypes of Vibrio parahaemolyticus isolates from clinical and seafood samples], Wei Sheng Wu Xue Bao 47, 508-511.

[81] Jiang, L., Ke, C., Xu, J., Chen, J., Chen, X., Chen, N., Shuai, J., and Fang, W. (2007) Listeria monocytogenes mutants carrying Newcastle disease virus F gene fused to its actA and plcB: in vitro expression and immunogenicity in chickens, Acta Biochim Biophys Sin (Shanghai) 39, 57-66.

[82] Chen, X., Xu, J., Shuai, J., Chen, J., Zhang, Z., and Fang, W. (2007) The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimurium, Int J Food Microbiol 115, 307-312.

[83] Chen, J. S., Jiang, L. L., and Fang, W. H. (2007) [Virulence determinants and its evolution of the genus Listeria], Wei Sheng Wu Xue Bao 47, 738-742.

[84] Yang, Z. Z., Fang, W. H., and Habib, M. (2006) First results of detection of PRRSV and CSFV RNA by SYBR Green I-based quantitative PCR, J Vet Med B Infect Dis Vet Public Health 53, 461-467.

[85] Xu, J. J., Jiang, L. L., Chen, N., Shuai, J. B., and Fang, W. H. (2006) [Characterization of a recombinant Listeria monocytogenes strain containing the fusion protein gene of Newcastle disease virus], Wei Sheng Wu Xue Bao 46, 445-450.

[86] Li, X. L., Shuai, J. B., and Fang, W. H. (2006) Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk, J Zhejiang Univ Sci B 7, 922-928.

[87] Li, L., Fang, W., Li, J., Huang, Y., and Yu, L. (2006) Oral DNA vaccination with the polyprotein gene of infectious bursal disease virus (IBDV) delivered by the attenuated Salmonella elicits protective immune responses in chickens, Vaccine 24, 5919-5927.

[88] Jiang, L. L., Xu, J. J., Chen, N., Shuai, J. B., and Fang, W. H. (2006) Virulence phenotyping and molecular characterization of a low-pathogenicity isolate of Listeria monocytogenes from cow"s milk, Acta Biochim Biophys Sin (Shanghai) 38, 262-270.

[89] Fang, W., Budde, B. B., and Siegumfeldt, H. (2006) Leucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes, FEMS Microbiol Lett 258, 208-213.

[90] Yang, Z. G., Sun, H. X., and Fang, W. H. (2005) Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice, Vaccine 23, 5196-5203.

[91] Jiang, L. L., Song, H. H., Chen, X. Y., Ke, C. L., Xu, J. J., Chen, N., and Fang, W. H. (2005) Characterization of a mutant Listeria monocytogenes strain expressing green fluorescent protein, Acta Biochim Biophys Sin (Shanghai) 37, 19-24.

[92] Chen, X. Y., John, M. D., Jiang, L. L., Shuai, J. B., and Fang, W. H. (2005) [Stability of recombinant plasmids in attenuated Salmonella typhimurium and their effects on bacterial invasiveness and multiplication], Wei Sheng Wu Xue Bao 45, 744-747.

[93] Li, L., Fang, W. H., Fan, Y. J., Xu, J., Fang, L., Li, J. R., and Yu, L. (2004) [Expression of the infectious bursal disease virus polyprotein in Vero cells using attenuated Salmonella typhimurium as transgenic carrier], Sheng Wu Gong Cheng Xue Bao 20, 437-440.

[94] Fang, W., Siegumfeldt, H., Budde, B. B., and Jakobsen, M. (2004) Osmotic stress leads to decreased intracellular pH of Listeria monocytogenes as determined by fluorescence ratio-imaging microscopy, Appl Environ Microbiol 70, 3176-3179.

[95] Liang, X. Y., Fang, W. H., and Jiang, L. L. (2003) [Safety, stability and immunogenicity of an oral DNA vaccine against Newcastle disease], Sheng Wu Gong Cheng Xue Bao 19, 24-29.

[96] Fang, W., Shi, M., Huang, L., Chen, J., and Wang, Y. (1996) Antagonism of lactic acid bacteria towards Staphylococcus aureus and Escherichia coli on agar plates and in milk, Veterinary research 27, 3-12.

[97] Fang, W., Shi, M., Huang, L., Shao, Q., and Chen, J. (1993) Growth of lactobacilli, Staphylococcus aureus and Escherichia coli in normal and mastitic milk and whey, Vet Microbiol 37, 115-125.

学术论文（中文）：

[1]方维焕,梁雪芽.减毒沙门氏菌为载体在Vero细胞中表达新城疫病毒融合蛋白[J].生物化学与生物物理学报,2002,04:488-493.

[2]柯春林,方维焕,马有智.硫醇活性胆固醇结合细胞溶素的结构与功能研究[J].动物医学进展,2002,05:25-27.

[3]梁雪芽,方维焕,江玲丽.鸡新城疫口服DNA疫苗的安全性、稳定性与免疫效力[J].生物工程学报,2003,01:24-29.

[4]张晓峰,方维焕,江玲丽,杜华华.应用聚合酶链反应检测食品中单核细胞增多性李斯特菌[J].中华预防医学杂志,2003,03:55-57.

[5]谢荣辉,方维焕,李建荣,卢觅佳,于涟.萧山鸡IL-2基因真核表达载体构建及其在Vero细胞中的表达[J].浙江大学学报(农业与生命科学版),2003,06:62-67.

[6]方维焕,张晓峰,StephenP.Oliver.乳铁素参与乳房链球菌对乳腺上皮细胞的粘附[J].中国兽医学报,2003,01:35-37.

[7]马有智,方维焕,柯春林,张晓峰.猪链球菌2型JX分离株的生物学特性[J].中国兽医学报,2003,04:326-328.

[8]马有智,方维焕.猪链球菌血清2型JX02株溶血素基因的克隆及序列分析[J].中国兽医学报,2003,05:460-462.

[9]方维焕,梁雪芽,李建荣.减毒沙门氏菌为载体传递DNA疫苗诱导对新城疫病毒的免疫保护[J].中国兽医学报,2003,06:527-530.

[10]李龙,方维焕,樊拥军,许健,方立,李建荣,于涟.减毒沙门氏菌为载体在Vero细胞中表达传染性法氏囊病病毒多聚蛋白基因[J].生物工程学报,2004,03:437-440.

[11]李肖梁,方维焕,马有智.鸭肝炎病毒Ⅰ型的分离及其鸡胚适应性[J].黑龙江畜牧兽医,2004,06:37-38.

[12]张晓峰,方维焕,程洁,施伟良.李斯特菌选择性增菌培养分离比较研究[J].中国公共卫生,2004,06:84-85.

[13]方维焕.动物源食品中病原微生物污染的监控[J].中国动物保健,2004,10:16-18.

[14]方维焕,裘志明.两种抗菌素油剂防治奶牛干奶期乳房炎的疗效比较[J].浙江农业大学学报,1990,03:63-66.

[15]方维焕,胡松华,刘汉儒,晏海涛.奶牛隐性乳房炎病原菌及其流行情况的调查[J].中国兽医科技,1991,06:17-18.

[16]方维焕.不同制备方法对中草药抗菌作用的影响[J].中兽医学杂志,1991,02:1-2.

[17]方维焕.几种中草药对中性白细胞吞噬功能的影响[J].中兽医学杂志,1991,01:5-7.

[18]方维焕,黄利权,胡松华,刘汉儒,蒋次升.奶牛隐性乳房炎的病原学调查(简报)[J].浙江农业大学学报,1992,01:21.

[19]方维焕,施明华.牛奶中大肠杆菌(Escherichiacoli)生长的荧光测定法[J].浙江农业大学学报,1992,04:62-65.

[20]蒋次昇,方维焕.略论《元亨疗马集》方剂的继承、发扬和创新——纪念《元亨疗马集》付梓380周年[J].中兽医医药杂志,1988,06:1-5.

[21]胡松华,方维焕,刘汉儒,蒋次升,朱平民,叶均安,叶定生,晏海涛.乳头药浴结合干奶期药物治疗控制奶牛乳房炎[J].中国兽医科技,1989,07:38-39.

[22]方维焕,朱平民.苄星邻氯青霉素的研制[J].浙江农业大学学报,1989,01:76-79.

[23]方维焕.兽医食品卫生学人才培养之浅见[J].动物保健,2006,06:16-17.

[24]杨宗照,方维焕.猪瘟合并猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)感染[J].中国兽医学报,2006,03:240-242.

[25]倪征,方维焕,张玉颖,云涛,陈锦清,刘光清.兔病毒性出血症病毒中国株(JX/CHA/97)全基因组分子克隆及序列分析[J].中国预防兽医学报,2006,06:668-671+675.

[26]蒋次升,方维焕,朱平民,胡松华.中西医结合防治奶牛隐性乳房炎[J].中国兽医科技,1994,08:32-34.

[27]方维焕,蒋成东,李央群,施明华.荧光法快速鉴定大肠杆菌的研究（简报）[J].浙江农业大学学报,1994,04:378.

[28]施明华,方维焕,李央群,蒋成东.荧光测定法在药敏试验中的应用[J].浙江农业学报,1995,06:54-59.

[29]戴慧,方维焕.杭州市外来生猪口蹄疫免疫抗体水平监测[J].浙江畜牧兽医,2012,04:34-35.

[30]康跃,梁权,方维焕,李肖梁.中华鳖TLR2部分序列的克隆及其组织表达差异分析[J].中国兽医科学,2012,09:958-964.

[31]武晓林,方维焕,俞盈,宋厚辉.结核分枝杆菌同源重组基因敲除系统的构建和应用[J].微生物学报,2012,09:1151-1159.

[32]陈皓,方维焕,田春庄.虫媒病毒性脑炎的ELISA诊断方法研究[J].中国畜牧兽医文摘,2012,07:51-52.

[33]黄丽,方维焕,俞盈,宋厚辉.基于光激活定位显微镜的膜蛋白鉴定技术[J].生物工程学报,2012,11:1378-1387.

[34]方维焕.棕曲霉分离菌的培养产物引起的猪霉菌毒素中毒的研究[J].浙江畜牧兽医,1981,03:44-45.

[35]单颖,张亦凯,程昌勇,方春,彭金荣,方维焕.斑马鱼胚胎的简易无菌培养体系及单增李斯特菌感染试验[J].微生物学报,,:1-14.

[36]王淑娜,方维焕.荧光定量PCR法检测副溶血弧菌tdh基因的表达差异[J].畜牧与兽医,2008,09:9-13.

[37]扈鸿霞,方维焕,刘建华,冉多良.PRRSVGP5基因的原核表达及其免疫性分析[J].动物医学进展,2008,05:32-35.

[38]陆承平,方维焕,陈金顶,廖明.兽医微生物学学科发展[J].中国家禽,2008,09:31-34.

[39]戴贤君,方维焕.减毒沙门氏菌为载体的草鱼口服生长抑素DNA疫苗的构建及其稳定性[J].中国兽医学报,2005,04:356-358.

[40]戴贤君,方维焕.减毒沙门氏菌为载体的口服生长抑素DNA疫苗对草鱼的安全性[J].中国兽医学报,2005,05:466-469.

[41]杨宗照,方维焕.PCV2的PCR鉴定及其相关序列分析[J].浙江农业科学,2005,06:76-78.

[42]柯春林,方维焕.单增李斯特菌生物膜及其形成机制的研究进展[J].中国微生态学杂志,2011,06:574-577.

[43]刘明远,刘全,方维焕,尹继刚,王学林,李建华,姜宁,张西臣,白雪,吴秀萍.我国的食源性寄生虫病及其相关研究进展[J].中国兽医学报,2014,07:1205-1224.

[44]梁雪芽,方维焕.猪生殖与呼吸综合征病毒的分子生物学研究进展[J].中国兽医科技,2001,08:14-17.

[45]马有智,方维焕,徐海圣.携带猪链球菌溶血素基因真核表达载体的减毒沙门氏菌的构建[J].江苏农业学报,2013,04:915-917.

[46]施明华,方维焕.荧光法快速鉴定大肠杆菌的初步研究[J].浙江农业学报,1999,02:38-41.

[47]章红兵,方维焕.猪链球菌多价油乳剂灭活苗对母猪免疫的效果观察[J].黑龙江畜牧兽医,2007,04:89-90.

[48]陈健舜,江玲丽,方维焕.李斯特菌毒力因子及其进化[J].微生物学报,2007,04:738-742.

[49]金培婕,吴蓓蓓,王淑娜,俞盈,钱永华,方维焕.浙江沿海地区海产品及环境中副溶血弧菌的分离与主要毒力基因分析[J].微生物学通报,2009,07:962-967.

[50]朱炳林,李俊,方维焕,李肖梁.中华鳖MyD88部分序列克隆及其在组织中的表达差异分析[J].水产学报,2010,07:1018-1024.

特邀报告：

1. Characterization of the gene locus encoding surface layer proteins in Lactobacillus crispatus isolate ZJ001 having antagonistic activity against enteropathogens. The 6th International Conference on Global Research and Education, September 26-30, 2007. Hamamatsu, Japan:

2. 食源性病原溯源与风险评估，上海市畜牧兽医学会兽医公共卫生学分会成立暨兽医公共卫生国际论坛，2009年9月28-29日，上海。

3. 食源性病原溯源技术: PFGEvs MLST，中澳畜牧兽医技术研讨会，2009年9月17-18日，陕西杨凌。

4. 我国的肉品安全问题：挑战与机遇，2009ASAS-CAAV亚洲及环太平洋地区动物科学技术大会(Inaugural ASAS-CAAV Asia Pacific Rim Conference)，2009年11月8-10日。

5. 单增李斯特菌抗酸应激与感染生物学，中国畜牧兽医学会兽医食品卫生学分会第十一次学术交流会大会报告，2010年4月23-24日，陕西杨凌。

6. Internalin profiling, multilocus sequence typing and virulence assenments suggest evolutionary history of theListeria monocytogenes-Listeria innocua clade. Oral presentation at the International Symposium on Problems of Listeriosis, May 5-8, 2010, Porto, Portugal.

7. Zoonotic foodborneListeria monocytogenes: Acid tolerance and infection, invited speech at XIII International Congress of Bacteriology and Applied Microbiology, September 6-10, 2011, Sapporo, Japan.

8. Use of antibiotics in animals and antimicrobial resistance. Joint Symposium on Antimicrobial Resistance, European University Centre at Peking University, Beijing, October 29, 2011.

获奖：

1. 鸡传染性法氏囊病基因免疫研究  浙江省科技成果一等奖（2002），第四完成人；

2. 集约化猪场猪链球菌病防治技术研究，浙江省科技成果三等奖（2003），第一完成人。

3. 食品（茶叶、海产鱼虾）安全关键技术研究与综合示范，浙江省科技成果二等奖（2006），第五完成人。

4. 宁波地区重大动物疫病防控关键技术研究，宁波市科学技术进步二等奖（2010），第二完成人  

专利：

1. 方维焕，陈健舜，江玲丽，李肖梁，王淑娜：李斯特菌种别及毒力快速检测试剂盒,专利号：200710066985.8

2. 方维焕，李肖梁，陈健舜，田国明，马有智：猪链球菌2型三重PCR快速检测试剂盒，专利号：ZL200610051960.6

3. 方维焕、徐程、江玲丽：鉴别猪口蹄疫免疫动物与感染动物的ELISA试剂盒，专利号：ZL200710164814.9

4. 李肖梁，方维焕：抗嗜水气单胞菌免疫球蛋白蛋黄粉包膜即制备方法，专利号：ZL200610052057.1

学术著作：

1. The Bovine Udder and Mastitis，芬兰赫尔辛基大学出版社1995，参编

2. 汉英中兽医辞典（修订本），中国农业出版社2003，编者

3. 兽医公共卫生学，中国农业出版社2003，副主编

4. 动物性食品卫生学，高等教育出版社2005，参编

5. 食品检验检疫学，中国农业出版社2007，副主编.