导师简介

姓 名：程昌勇

职 称：副教授

科研方向：

1.食源性病原菌的应激生物学和致病机制；

2.病原与宿主互作调控机制；

3.动物性食品安全与公共卫生

个人简述：程昌勇，副教授，硕士生导师。博士毕业于浙江大学动物科学学院预防兽医学专业。现担任浙江农林大学动物科技学院兽医学科专任教师。主要从事重要动物疫病的分子流行病学，食源性病原微生物（以单增李斯特菌为主）的抗应激生物学、致病机制以及与宿主互作等领域研究。目前作为项目负责人共主持国家自然科学基金青年基金项目1项、浙江省公益技术应用研究项目1项、浙江省自然科学基金一般项目1项、浙江农林大学人才启动项目1项以及校企合作研究课题2项；作为子课题负责人参与“十三五”国家重点研发计划专项项目1项；作为主要参与人参加国家自然科学基金、浙江省自然科学基金等项目若干项。目前在国外期刊（Journal of Biological Chemistry, Frontiers in Microbiology, Scientific Reports等）上共发表SCI论文20余篇。

教育背景：

1.2005.09-2009.06：安徽科技学院，动物医学专业，本科

2.2009.09-2014.06：浙江大学，预防兽医学，博士（硕博连读）

工作经历：

1.2014.07-2016.12：浙江农林大学动物科技学院，讲师

2.2017.01-至今：浙江农林大学动物科技学院，副教授

学术兼职：担任Folia microbiologica、PLoS One、Frontiers in Microbiology等SCI期刊审稿人。

科研项目：

1.“单核细胞增多性李斯特菌硫氧还蛋白TrxA介导的抗氧化及鞭毛形成机制研究”（9万，浙江省自然科学基金一般项目，立项号：LY17C180001，主持）

2.“精氨酸抑制因子ArgR与应激调控因子SigB介导的单增李斯特菌抗酸应激调控机制研究”（25万，国家自然科学基金，立项号：31402215），主持

3.“食品和动物饲料中四种霉菌毒素的精准检测技术开发及应用”（15万，浙江省公益项目），主持

4.“西式风干发酵肉制品的开发”（13.68万，浙江青莲食品股份有限公司），主持

5.“冷鲜肉物流货架期与质量安全溯源系统开发及应用示范检测”（13.68万，浙江青莲食品股份有限公司），主持

6.单增李斯特菌精氨酸抑制因子ArgR调控抗酸应激机制研究（5万，浙江农林大学人才启动项目），主持

7.“硫氧还蛋白TrxA介导的单增李斯特菌运动性和抗氧化应激机制研究”（83万，国家自然科学基金，立项号：31470179），主要参与人

8.单核细胞增多性李斯特菌代谢物控制蛋白CcpA介导的胞内pH稳态调控机制研究（21万，国家自然科学基金，立项号：31502083），主要参与人

9.与死亡结构域蛋白TRADDFADDRIP1互作的牛分枝杆菌蛋白的鉴定和功能研究（20万，国家自然科学基金，立项号：31502034），主要参与人

10.“鲱精胺脱亚胺酶代谢通路对单增李斯特菌抗酸应激能力的影响及其分子作用机制”（国家自然科学基金，立项号：31101829），主要参与人

11.“食品中微生物毒素精准检测技术”（国家高技术研究发展计划(863计划)，立项号：2012AA101602）

12.“1,3-二磷酸甘油衍生物在革兰氏阳性胞内模式菌的膜锚定”（国家自然科学基金，立项号：31272570），主要参与人

主要论文（英文）

1.Cheng C, Dong Z, Han X, Sun J, Wang H, Jiang L, Yang Y, Ma T, Chen Z, Yu J, Fang W and Song H (2017) Listeria monocytogenes10403S Arginine Repressor ArgR Finely Tunes Arginine Metabolism Regulation under Acidic Conditions. Front. Microbiol. 2017. 8:145. doi: 10.3389/fmicb.2017.00145. (SCI, IF 4.165)

2.Cheng, C., X. Wang, Z. Dong, C. Shao, Y. Yang, W. Fang, C. Fang, H. Wang, M. Yang, L. Jiang, X. Zhou, and H. Song, Aminopeptidase T of M29 Family Acts as A Novel Intracellular Virulence Factor for Listeria monocytogenes Infection. Scientific Reports, 2015. 5: p. 17370. (SCI, IF 5.786)

3.Cheng, C., Y. Yang, Z. Dong, X. Wang, C. Fang, M. Yang, J. Sun, L. Xiao, W. Fang, and H. Song, Listeria monocytogenes varies among strains to maintain intracellular pH homeostasis under stresses by different acids as analyzed by a high-throughput microplate-based fluorometry. Front Microbiol, 2015. 6: p. 15. (SCI, IF 3.941)

4.Cheng, C., Chen, J., Fang, C., Xia, Y., Shan, Y., Liu, Y., Wen, G., Song, H., and Fang, W. (2013) Listeria monocytogenes aguA1, but not aguA2, encodes a functional agmatine deiminase: Biochemical characterization of its catalytic properties and roles in acid tolerance. J Biol Chem 288, 26606-26615. (SCI, IF 4.651)

5.Cheng, C., Chen, J., Shan, Y., Fang, C., Liu, Y., Xia, Y., Song, H., and Fang, W. (2013) Listeria monocytogenes ArcA contributes to acid tolerance. J Med Microbiol 62, 813-821. (SCI, IF 2.297)

6.Shan, Y., C. Fang, C. Cheng, Y. Wang, J. Peng, and W. Fang, Immersion infection of germ-free zebrafish with Listeria monocytogenes induces transient expression of innate immune response genes. Front Microbiol, 2015. 6: p. 373. (SCI, IF 3.941)

7.Hu QL, Wu M, Fang C, Cheng CY, Zhao MM, Fang WH, et al. Engineering Nanoparticle-Coated Bacteria as Oral DNA Vaccines for Cancer Immunotherapy. Nano Lett. 2015;15(4):2732-9. doi: 10.1021/acs.nanolett.5b00570. PMID: WOS:000352750200079. (IF=13.779)

8.Fang, C., T. Cao, C. Cheng, Y. Xia, Y. Shan, Y. Xin, N. Guo, X. Li, H. Song, and W. Fang, Activation of PrfA results in overexpression of virulent factors but does not rescue the pathogenicity of Listeria monocytogenes M7. J Med Microbiol, 2015. (SCI, IF 2.297)

9.Fang, C., Y. Shan, T. Cao, Y. Xia, Y. Xin, C. Cheng, H. Song, X. Li, and W. Fang, Prevalence and Virulence Characterization of Listeria monocytogenes in Chilled Pork in Zhejiang Province, China. Foodborne Pathog Dis, 2015. (SCI, IF 1.905)

10.Fang, C., T. Cao, Y. Shan, Y. Xia, Y. Xin, C. Cheng, H. Song, J. Bowman, X. Li, X. Zhou, and W. Fang, Comparative genomic analysis reveals that the 20K and 38K prophages in Listeria monocytogenes serovar 4a strains Lm850658 and M7 contribute to genetic diversity but not to virulence. J Microbiol Biotechnol, 2015. (SCI, IF 1.525)

11.Wen, G., Zhang, Y., Zhang, X., Hu, H., Zhang, H., Cheng, C., Wang, X., Li, X., and Fang, W. (2013) Functional characterization of porcine LSm14A in IFN-beta induction. Vet Immunol Immunopathol 155, 110-116. (SCI, IF 1.877)

12.Chen, J., Chen, F., Cheng, C., and Fang, W. (2013) Prevalence of the lmo0036-0043 gene cluster encoding arginine deiminase and agmatine deiminase systems in Listeria monocytogenes. New Microbiol 36, 187-192. (SCI, IF 1.677)

13.Chen, J., Cheng, C., Lv, Y., and Fang, W. (2012) Genetic diversity of internalin genes in the ascB -dapE locus among Listeria monocytogenes lineages III and IV strains. J Basic Microbiol 53, 778-784 (SCI, IF 1.198)

14.Chen, J., Fang, C., Zhu, N., Lv, Y., Cheng, C., Bei, Y., Zheng, T., and Fang, W. (2012) Genetic organization of ascB-dapE internalin cluster serves as a potential marker for Listeria monocytogenes sublineages IIA, IIB, and IIC. J Microbiol Biotechnol 22, 575-584. (SCI, IF 1.399)

15.Zhao, H., Chen, J., Fang, C., Xia, Y., Cheng, C., Jiang, L., and Fang, W. (2011) Deciphering the biodiversity of Listeria monocytogenes lineage III strains by polyphasic approaches. J Microbiol 49, 759-767. (SCI, IF 1.276)

16.Jiang, J., Chen, J., Cheng, C., Hu, H., Bai, F., Chen, N., Yan, G., and Fang, W. (2011) Disruption of InlC2 enhances the internalization of Listeria monocytogenes by epithelial cells. World Journal of Microbiology and Biotechnology 27, 2155-2161. (SCI, IF 1.262)

17.Chen, J., Xia, Y., Cheng, C., Fang, C., Shan, Y., Jin, G., and Fang, W. (2011) Genome sequence of the nonpathogenic Listeria monocytogenes serovar 4a strain M7. J Bacteriol 193, 5019-5020. (SCI, IF 3.194)

18.Chen, J., Cheng, C., Xia, Y., Zhao, H., Fang, C., Shan, Y., Wu, B., and Fang, W. (2011) Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance. Microbiology 157, 3150-3161. (SCI, IF 2.852)

19.Chen, J., Chen, Q., Jiang, L., Cheng, C., Bai, F., Wang, J., Mo, F., and Fang, W. (2010) Internalin profiling and multilocus sequence typing suggest four Listeria innocua subgroups with different evolutionary distances from Listeria monocytogenes. BMC Microbiol 10, 97. (SCI, IF 3.104)